HLA B - 27 BY FLOWCYTOMETRY

The Human Leukocyte Antigen HLA system is a group of genes located on chromosome 6 that plays a vital role in immune system regulation. HLA proteins help the body recognise its own cells and distinguish them from harmful microorganisms. HLA-B27 belongs to the Human Leukocyte Antigen HLA system group of proteins that help the immune system distinguish between the body's own cells and foreign substances such as bacteria and viruses.

The test is performed when patients have

• Chronic lower back pain
• Morning stiffness
• Sacroiliac joint pain
• Persistent joint inflammation
• Eye inflammation (uveitis)
• A family history of ankylosing spondylitis
• The recurrent inflammatory arthritis

What is the HLA-B27 Test?

The HLA-B27 Test is a specialised laboratory blood test that is used to detect the presence of Human Leukocyte Antigen B27 HLA-B27 protein on the surface of white blood cells. The HLA-B27 Test is used as a supportive diagnostic tool and is interpreted together with the patient's symptoms, physical examination, imaging studies and other laboratory investigations.

What is the Procedure of HLA-B27 Test?

• The skin over a vein is cleaned with an antiseptic.
• A sterile needle is inserted into a vein in the arm.
• A blood sample is collected into a laboratory tube.
• The needle is removed after sample collection.
• Gentle pressure is applied to prevent bleeding.
• The sample is labelled correctly.
• The specimen is transported to the laboratory.
• Its specialised laboratory techniques detect the presence or absence of the HLA-B27 antigen.
• Quality control procedures are completed.

What is the Purpose of HLA-B27 Test?

The HLA-B27 Test is performed for several important clinical purposes:

• To assist in diagnosing ankylosing spondylitis.
• To evaluate suspected axial spondyloarthritis.
• To investigate chronic inflammatory back pain.
• To diagnose reactive arthritis.
• To assess psoriatic arthritis.
• To evaluate juvenile idiopathic arthritis.
• To investigate unexplained joint inflammation.
• To support diagnosis of acute anterior uveitis.
• To assess recurrent inflammatory eye disease.
• To evaluate patients with a family history of HLA-B27-associated disorders.
• To complement imaging studies.
• To support rheumatological evaluation.
• To assist in treatment planning.
• To evaluate autoimmune joint disorders.
• To improve diagnostic accuracy.

What are the Benefits of the HLA-B27 Test?

• It helps to identify genetic susceptibility.
• Supports early diagnosis of autoimmune disorders.
• Assists rheumatologists in clinical evaluation.
• It is useful for investigating chronic inflammatory back pain.
• It helps differentiate inflammatory from mechanical joint disease.
• Complements imaging studies.
• Can guide further investigations.
• It supports personalised treatment planning.
• It provides valuable diagnostic information when combined with clinical findings.

What are the Conditions Associated with HLA-B27?

What are the Limitations of the HLA-B27 Test?

The following are some important limitations of the HLA-B27 Test:

• A positive result does not confirm disease.
• Many healthy people are HLA-B27 positive.
• A negative result does not completely exclude autoimmune disease.
• Clinical symptoms remain essential for diagnosis.
• Imaging studies are usually required.
• Laboratory reference methods may vary.
• Additional blood tests may still be necessary.
• The test cannot determine disease severity.
• It does not predict exactly who will develop disease.
• Its results must always be interpreted by a qualified healthcare professional.

Who Should Undergo the HLA-B27 Test?

Healthcare providers may recommend the HLA-B27 Test for individuals who:

• It experiences morning stiffness that improves with physical activity.
• Have chronic pain in the sacroiliac joints.
• Develop unexplained swelling and pain in large joints.
• Have recurrent episodes of eye inflammation (acute anterior uveitis).
• Have reactive arthritis following a bacterial infection.
• Have psoriasis associated with joint pain.
• They have inflammatory bowel disease with joint symptoms.
• It is suspected of having axial spondyloarthritis.
• I have juvenile idiopathic arthritis.
• Need supportive testing during rheumatological evaluation.

What is the Preparation before the Test?

The HLA-B27 Test requires minimal preparation.

• Fasting is not required before the test.
• Drink adequate water before blood collection.
• Continue your regular medications unless instructed otherwise.
• Inform your healthcare provider about all medications and supplements you are taking.
• Mention any previous diagnosis of autoimmune disease.
• Inform the physician about any recent infections or inflammatory illnesses.
• Carry previous laboratory reports and imaging studies if available.
• Follow any additional instructions provided by your healthcare provider.

Where to Book Online Tests for HLA-B27 in Delhi?

In Delhi there are many diagnostic centres to book online tests for HLA-B27 but patients prefer City Imaging for the best results and at a very affordable price.

Which HLA-B27 centre is near me in Delhi?

HLA-B27 near me in Delhi is City Imaging and Clinical Labs. This centre provides the best results and the rates of the tests are very affordable as compared to others.

How to book a HLA-B27 Flowcytometry test online at City Imaging?

Patients can book the test HLA-B27 Flowcytometry online at City Imaging by visiting the website and book the test appointment online. There are also customer care numbers on the website that the patient can dial and book our test. In Delhi there are many diagnostic centres to book online tests for HLA-B27 but patients prefer City Imaging for the best results and at a very affordable price.

What is the HLA-B27 price in Delhi?

HLA-B27 price in Delhi may range from INR 1800 to INR 3500. The price may vary depending on various factors like location of diagnostic centres. It is expensive because of the advanced equipment and the skilled professionals used for this test.

Why to Choose City Imaging for HLA-B27 test in Delhi?

In Delhi patients choose City Imaging for the HLA-B27 test because this centre has the best machines that give the best results. The diagnostic centre has very good patient service with helpline numbers for round the clock availability.

Final Words

The HLA-B27 by Flowcytometry Test is a valuable genetic blood test that detects the presence of Human Leukocyte Antigen B27 protein marker strongly associated with several autoimmune and inflammatory disorders. The presence of HLA-B27 increases the likelihood of these conditions; it is not a diagnostic test by itself. Many healthy individuals carry the HLA-B27 protein without ever developing disease while some patients with autoimmune disorders test negative. Therefore, healthcare professionals always interpret results together with the symptoms, physical examination laboratory findings and imaging studies. Early identification of HLA-B27-associated conditions allows timely treatment helping reduce pain, prevent complications, preserve joint function and improve overall quality of life. Regular medical follow up and appropriate treatment remain essential for achieving the best long term outcomes.

Frequently Asked Questions (FAQ)

What is the HLA-B27 Test?

It is a blood test that detects the presence of HLA-B27 protein on white blood cells.

Why is the HLA-B27 Test performed?

It helps to support the diagnosis of autoimmune inflammatory diseases such as ankylosing spondylitis and reactive arthritis.

Is fasting required before this test?

No fasting is required.

Does positive HLA-B27 result mean you have ankylosing spondylitis?

No, positive results only indicate increased genetic susceptibility and must be interpreted together with the symptoms and other investigations.

What are other names of HLA-B27 Test?

The other names of the HLA-B27 Test are HLA B- 27 BY FLOWCYTOMETRY